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15th
World Congress Clinical Nutrition
19th
– 22nd September 2010 El Sokhna Resort - Egypt
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Copyright © 2010.
WCCN2010.COM
All rights reserved |
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Intestinal permeability and
fecal microbiota among apparently
healthy adolescents and the impact
of nutritional intervention with
fermented or non-fermented
supplements .
Laila Hussein,
Nahed Hussein1, Mahmoud
Mohammed Department of Nutrition,
National research Center, Giza and
1) Department of
Nutrition, Faculty of special
education, Ain Shams Univ
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Background: The
gastrointestinal (GI) epithelium is
continuously exposed to numerous
chemical, physical and biological
insults such as resident bacteria
and intestinal pathogens and their
products. The defensive mucus
blanket provides a formidable
barrier between the mucosal
epithelium and the lumen of the GI
tract and its contents .
Gastrointestinal mucin is a
constituent of luminal tight
junction barrier function [TJ] and
is the first line of host defense
against invading pathogens.
Toll-like receptors on the surface
of epithelial cells play a role in
maintaining epithelial barrier
function in response to enteric
pathogens and parasites. Increase in
intestinal permeability is an
important etiologic factor of
inflammation of the proximal small
bowel , which is common in countries
with poor hygienic conditions and
where coinfections with multiple
enteric pathogens are prevalent.
Tumour necrosis factor [TNF-]
increases intestinal TJ permeability
and had been reported to be a
contributing factor of intestinal
permeability defect.
Selected foods, or their components,
offer benefits beyond basic
nutrition and this knowledge
continues to be supported by
research that demonstrates that
dietary habits are intrinsically
linked to health promotion and
disease prevention. The intake of
fermented foods and of polyphenolic
- rich foods have been expected to
be important materials for the
maintenance of health. Such foods
have usually antioxidative,
anti-inflammatory and antimutagenic
activities.
Objective:
Measuring in vivo intestinal
permeability among apparently
healthy adolescents. Improving the
intestinal permeability through
nutritional intervention programs
based on supplements with either
fermented food or polyphenol – rich
caccoa beverage.
Design of the experiment: Apparently
healthy males (11 – 14 years of age)
living under poor hygienic
conditions were openly recruited. A
3-week, randomised, double-blind,
placebo-controlled trial, in 4
parallel groups in participants with
leaky gut having high baseline
lactulose : mannitol ratios was
conducted. Thirty two participants
were randomised to 4 groups: [a]
sobya [ fermented rice-based
porridge]; [B] appropriate placebo
porridge; [C] Caccoa beverage or [D]
placebo beverage. To assess
intestinal permeability, 5-hour
urine collections were obtained from
each subject following the ingestion
of a solution (2 ml kg-1 body
weight) containing 200 mg of
lactulose and 50 mg of mannitol
after an overnight fast. Urinary
lactulose and mannitol were
determined by gas–liquid mass
spectrometry. The composition of
fecal microbiota and fecal enzyme
activity were also measured. The
assessment of bacterial enzyme
activities was used to demonstrate
diet-related changes in the colon .
Blood samples were collected for
assaying selected biomarkers of
health status. All of the data
measured were analyzed
statistically.
Results Initially,
high ratio of urinary lactulose
mannitol was found among the
majority of the subjects, which
exceeded the standard norms. The
consumption of Lactobacillus
acidophilus (L1a) or the traditional
sobya supplement were equally
efficient in lowering the L:M ratio
among all subjects to almost normal
level. Caccoa drinking didn't affect
the intestinal permeability to any
extent . The total microbial counts
of fecal samples showed that at the
end of 21 days intervention with
sobya, Lactobacilli total count
increased by 100 up to 10 thousand
fold .
Conclusion The
results of this study clearly
demonstrate that the power of
simple, low-technical and low-cost
interventions is comparable to the
high tech commercial products in its
efficiency in restoring the gut
barrier function. It is superior
than the commercial brand on the
basis that it is accessible to the
majority of the population.
Incentives for healthy ways of
living should be provided to assure
changing lifestyle . Scientific
evidences prove that changing of
life style may be associated with
the expression of quite few
beneficial genes. Characterization
of the molecular mechanism
underlying the positive response to
intervention with sobya is under
investigation.
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