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15th
World Congress Clinical Nutrition
19th
– 22nd September 2010 El Sokhna Resort - Egypt
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Copyright © 2010.
WCCN2010.COM
All rights reserved |
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Chemistry, hepatoprotective ,
anticancer activities of Omega – 3
rich preparations purified from the
seed oil of Linum grandiflorum Desf.
Magdy Mohammed*,1,2,3,
Ming Chen 4, Lin Zhai
4, Nabaweya Ibrahim3,
Sanaa Ibrahim5
1Danish Inst Agric Sc
(DIAS), Depart Food Sc, Rersearch
Center Aarslev, Aarhus Univ,
Denmark.
2Nucleic Acid Center,
Inst Physics & Chemistry, Univ
Southern Denmark, Denmark.
4 Depart Clinical
Microbiol, Univ Hosp Copenhagen, ,
Denmark.
3Departments
Pharmacognosy & 5Therapeutic
Chemistry, National Research Center,
Egypt.
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Aim of work: To
characterize the composition of the
oily fraction of the seed meal of
the medicinal plant Linum
grandiflorum Desf. (Linaceae) and to
test its cytotoxicity against EL4 (Murine
Leukemia) and its hepatoprotective
activity.
Materials: Linum
grandiflorum Desf. were collected in
March 2006, from El-Orman Garden,
Giza Governorate, Egypt, and kindly
identified by The Head of
Specialists of Plant Taxonomy at the
garden, Miss. Tressa Labib. The
seeds of the plant were collected
and kept for further investigation.
A voucher specimen (No. 38) of the
whole plant was kept at the
Herbarium of National Research
Center (HNRC).
Methods: The seed
meal (184.46 g) was subjected to
extraction with MeOH (40 ºC) then
the MeOH extract was fractionated
with petroleum ether (60 – 80 ºC).
The resulted petroleum ether
fraction was evaporated under
reduced pressure till completely
dryness, to afford a crude oily
fraction (11.67 g, 6.33%) which was
washed several times with MeOH to
remove all the polar contaminants.
Biological study to assess
the Hepatoprotective effect:
Female Wister Rats 150-160 gm,
1ml/kg body wt. three times per week
for 6 wks. Study was carried out on
the serum e.g., Liver Function (AST,
ALT, ALP, Total Protein,
Phospholipids, and Cholesterol).
Histological & Histopathological
Liver, Kidney and Spleen, and to
evaluate the damage caused by CCL4
and the role of the seed oil of
Linum grandiflorum Desf. to improve
all the Liver parameters.
Anticancer activity:
The cell line EL4 (Murine Leukemia)
was purchased from National Cancer
Institute (NCI). A standard
high-flux anticancer-drug screening
method using Sulforhodamine B (SRB)
assay (Skehan et al., 1990, 82,
1107–1112).
Laboratory investigations:
The instrumentations of
matrix-assisted laser desorption and
ionization time-of-flight/mass
spectrometry (MALDI-TOF/MS),
electro-spray ionization/mass
spectrometry (ESI/MS), 1D- and
2D-NMR spectroscopy, were used to
characterize the seed oil
constituents.
Results: The
analysis demonstrated that the seed
oil consisted mainly of TAG 54:9 (trilinolenyl-glycerol
18:3-18:3-18:3), TAG 54:8
(18:3-18:3-18:2) and TAG 54:7
(18:3-18:3-18:1). These TAGs contain
mainly α-linolenic acid (18:3)
(Omega-3); while they are poor in
linoleic acid (18:2) and oleic acid
(18:1) is in trace amounts.
Anticancer activity:
The cytotoxicity of the seed oil was
evaluated against EL4 (Murine
Leukemia) cell line, which resulted
with IC50 = 100 µg/mL.
Hepatoprotective activity:
A remarkable disturbances was
observed in the levels of all tested
parameters. On the other hand, rats
injected with the toxic agent and
left for one and half month to self
recover showed moderate improvements
in the studied parameters, while,
treatment with the seed oil
ameliorated the levels of the
disturbed biochemical parameters and
the Histopathological liver kidney
and spleen profiles. Our results
show that seed oil of Linum
grandiflorum Desf. under study
revealing a remarkable effect,
enhancing the levels of all tested
parameters in liver chemical
toxicity.
Conclusion: The
present study suggests that, the
seed oil of Linum grandiflorum Desf.
(Linaceae) can lend its self to
novel products in the pharmaceutical
industries as a new rich-source of
Omega – 3 with a significant
anticancer and hepatoprotective
activity.
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