15th World Congress Clinical Nutrition

19th – 22nd September 2010  El Sokhna Resort -  Egypt

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Effect of soybean dietary supplement and a blend of tea on gene transcription in mice liver cells
Hanaa S Oraby
Department of Cell Biology, NRC, Giza Egypt

Nutrigenomics studies revealed that individual compounds, as well as complex mixtures of chemicals, derived from food alter the expression of genes in the human body. Isoflavones found in tea and soybean has been suggested to contribute to protection from carcinogens.

Objectives: To evaluate the protective action of drinking tea blend or soybean isoflavonoids dietary supplementation against DNA damage induced by treatment with cyclophosphamide (CP). Study design and Laboratory investigation Quantitative analysis of the expression of GPX mRNA by reverse transcription polymerase chain reaction (RT-PCR) was performed to quantify the amount of GPX mRNA present in liver cells in response to the treatment with CP before and after feeding mice with soybean isoflavonoids dietary supplements. The role of drinking a blend of red, green and black tea on the regulation of transcriptional levels of GPX mRNA and its related modulator effects on CYP11B2 mRNA were also investigated.

Results showed that CP reduced the level of transcription of GPX compared to control. Feeding mice with basal diets supplemented with soybean isoflavonoids extracts (0.2% & 0.4%) enhanced the level of transcription of GPX compated to animals treated with a single dose of cyclophosphamide. Results also showed that drinking a 1% mixture of green, red and black tea for two weeks, as a sole source of drinking, up-regulated the expression of GPX mRNA significantly in mice pretreated with CP. A concomitant down-regulation of CYP11B2 mRNA was also recorded in association with the treatment. Individual variation in levels of gene transcription and the possible response to dietary components or drugs will be discussed.


   
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