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15th
World Congress Clinical Nutrition
19th
– 22nd September 2010 El Sokhna Resort - Egypt
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Copyright © 2010.
WCCN2010.COM
All rights reserved |
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Cysteine - Containing Compounds
Ameliorate Diabetic Complications in
Rat models
Zakia Abdelkader, Amal Hamza, Lamiaa
Barakat , Mai Abdelkawi
Biochemistry & Nutrition Depart-
Women’s College ; Ain Shams Univ.,
Cairo, Egypt
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Aim of the work:
This study was designed to
investigate the beneficial effect of
some cysteine containing compounds
on biochemical deterioration induced
by diabetes.
Design of the experiment:
Eighty male albino rats were divided
into 8 groups of 10 rats each. Group
(1) was set as healthy control,
Group (2) was set as diabetic
control. Groups 3 through 8 were set
as diabetic groups supplemented with
cysteine containing compounds in two
doses (1g or 2g per liter of
drinking water Groups (3 - 4)
supplemented with S-allyl cysteine,
Groups (5 - 6) supplemented with
S-methyl cysteine. Groups (7 - 8)
supplemented with N-acetyl cysteine.
Rats were set diabetic after
injection by alloxane and their
blood glucose levels reached 200mg
or higher.The experiment lasted for
6 weeks. At the end of the
experiment serum and tissue samples
were collected to perform the
biochemical analysis. Glucose,
insulin, malondealdehyde (MDA),
superoxide dismutase (SOD),
glutathione reductase (GSH), urea,
creatinine, alanine amino
transferase (ALT), asparatet amino
transferase (AST) and alkaline
phosphatase (ALP) were all measured
in serum. Lipid profile was also
measured in the serum Total protein
and the activities of glutathione
peroxidase (GPX), and catalase were
also measured in the kidney and
liver homogenate
Results Diabetic
rats supplemented with cysteine
containing compounds controlled the
blood glucose and insulin levels
significantly compared to the
respective values obtained with the
diabetic control. Parameters of the
liver and kidney functions were also
significantly improved in the groups
supplemented with CYS compared to
the respective mean values with the
diabetic control. The antioxidant
status showed also statistically
significant improvement.in the CYST
– supplemented groups over the
diabetic control group. Moreover
cysteine containing compounds showed
a significant hypolipdemic effect in
diabetic rats. The antioxidant
effect of NAC, SMC and L-Cys was
dose dependent.
The higher supplementation with
(NAC) or (SMC) [2g/ liter of
drinking water controlled the blood
glucose significantly . The lipids
profile levels and ALP activity were
also withing the normal values.
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