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15th
World Congress Clinical Nutrition
19th
– 22nd September 2010 El Sokhna Resort - Egypt
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Copyright © 2010.
WCCN2010.COM
All rights reserved |
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Carbohydrate and protein composition
of DICHROSTACHYS CINEREA L and
testing its anticancer activity
against hepatic and other cell
lines.
A. Abou Zeid* M.S. Hifnawy** and
R.S. Mohammed*
*Pharmacognosy Dept. National
Research Centre, Dokki, Cairo,
Egypt.
**Pharmacognosy Dept., Faculty of
Pharmacy, Cairo Univ., Cairo, Egypt
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Aim of the work: To
evaluate the bioactivity of the
components of DICHROSTACHYS CINEREA
as anticancer agent.
Method: The total
ethanol extract of the aerial parts
of Dichrostachys cinerea were
prepared, its structure was
elucidated and the extract was
tested biologically The
antimicrobial screening of the total
ethanol extract and successive
extractions of the plant were
investigated against gram +ve, gram
-ve bacteria, yeast and fungi
applying the diffusion assay
according to the antibiotic assay
disc method [2,3]. The cytotoxic
potency of the extract was tested
using the method of Skehan, et al
[1] against five human tumor cell
lines, namely Hepg2 (Liver carcinoma
cell line),Hela (Cervix carcinoma
cell line), U25l (Brain tumor cell
line), MCF7(Breast carcinoma cell
line) and H460 (Lung carcinoma cell
line) .
Results: The total
carbohydrates and free sugars
contents in the ethanol extract
averaged 4.24% and 2% , respectively
with IC50 of 4.3μg/dl and 2.41μg/dl
against Hepg2 and U251,
respectively. All extracts showed
moderate activities againest Gram +ve
and Gram –ve bacteria . The extracts
were however either inactive or with
moderate activity against yeast and
fungi. HPLC separation [5] of low
molecular weight carbohydrates
revealed that Maltose 33.21% and
galacturonic acid 21.41% were the
most prominent sugars. Sugars
liberated after hydrolysis of the
ethanol extract under cold (90.9 %)
or hot (91.0 %) conditions were
almost comparable. However, HPLC
separation of the cold hydrolyzate
showed ribose to be richest (55.7%);
followed by glucose 11.7%, fructose
8.5% and glucuronic acid 8.4% .
Ribose (35.8 %) was also highest
after HPLC separation; followed by
mannose (24.5%) and glactcuronic
acid 8.3%.
The plant is safe to animals since
LD50 of the total ethanol extract
was 6.9g /kg body weight. It can be
used as chemoprotective agent
against liver and brain carcinoma.
This plant with crude protein of
16.4% can be a rich plant protein
source.
Total and free amino acids content
separated 16 amino acids ; whereby
the essential amino acids made up
35.8% of the total protein ; and
24.0% of the free amino acids.
References
1.Skehan P, Storeng R, Scudiero D,
Monks A, McMahon J, Vistica D,
Warren JT, Bokesch H, Kenney S,
Boyd, MR (1990) J. Natl Cancer Inst.
82 : 1107 Gnanamanickam, S.S., and
Mansfield, J.W.: Phytochem., 20, 997
(1981).
3.Govindarajan, G. and Gannamanickam,
S.S.: Curr.Sci.,50, 1077 (1981
4.Dubois, M.; Gilles, K.A. ;Hamilton,J.K.
; Rebors ,P.A. and Smith ,F.,
Analytical Chem ., 28, 250 (1956).
5.Gertz,C.H.(1990).HPLC tips and
tricks.Great Britain at the add
press, oxford pp.(608).
6.Eppendorf–Germany–LC 3000 Amino
Acid Analyzer
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