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15th
World Congress Clinical Nutrition
19th
– 22nd September 2010 El Sokhna Resort - Egypt
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Copyright © 2010.
WCCN2010.COM
All rights reserved |
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The effects of Resveratrol and
Cilostazol administration on
oxidant/antioxidant status in flap
tissues of rats exposed to Nicotine
Aslihan Avci, Hakan Gence, Ebru
Gurleyik, Derya Oztuna, Ilker Durak
Ankara Univ. Medical Faculty
Department of Biochemistry, Turkey
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Objectives: In this
study, to analyze the harmful
effects of nicotine and beneficial
effects of resveratrol and
cilostazol on flap viability of rats
exposed to nicotine, the oxidant and
antioxidant parameters, DNA turnover
enzyme activities were examined.
Methods: Rats were
divided into four groups: Control
(Group 1), Nicotin (Group 2),
Nicotine and Resveratrol (Group 3),
Nicotine and Cilostazol (Group 4).
Subcutaneous nicotine injections of
2 mg/kg/day were administered to
rats in group 2, 3, and 4 for two
weeks preoperatively. Group 3 rats
received 40 mg/kg/day resveratrol
orogastricly for 7 days beginning
with surgery while Group 4 rats
received 100 mg/kg/day cilostazol
like group 3 treatment procedure. A
dorsal peninsula flap (McFarlane
flap) was planned on the rat dorsum.
Postoperative 7th day full thickness
biopsies were taken from necrotic
borders for analyses.
Malondialdehyde (MDA) levels,
superoxide dismutase (SOD), catalase
(CAT), glutathione peroxidase (GSH-Px),
xsantine oxidase (XO), and adenosine
deaminase (ADA) enzyme activities
were analyzed in the flap tissue.
Results: ADA and XO
enzyme activities showed a
statistically significant increase
in the groups 3 (p<0.05) and 4
(p<0.01) (resveratrol and cilostazol
administered groups) compared to
control group. In group 3, MDA
levels were found the lowest within
the groups although this was not
statically significant. Also for
GSH-Px, CAT, SOD there were no
differences between the groups
Conclusion:
According to decrease in MDA levels
in resveratrol administered group,
we suggested that resveratrol played
an antioxidant role on the flap
viability. Since ADA and XO are DNA
turnover enzymes, statistically
significant increased ADA and XO
enzyme activities that we found
might be due to increased cell
generation and DNA synthesis. These
findings explain the beneficial
effect of resveratrol on wound
healing.
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