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15th
World Congress Clinical Nutrition
19th
– 22nd September 2010 El Sokhna Resort - Egypt
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Copyright © 2010.
WCCN2010.COM
All rights reserved |
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Hepatoprotective activity of the
ethanolic extract of ASTRAEUS
HYYGROYGROMETRICUS (PERS.) MORG. and
TRICHOLOMA GIGANTEUM MASSEE
Gunjan Biswas1 and
Krishnendu Acharya1*
1Molecular and Applied
Mycology and Plant Pathology
Laboratory, Department of Botany,
University of Calcutta, , Kolkata ,
India
krish_paper@yahoo.com
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Objective: An
attempt has been made to evaluate
the hepatoprotective potency of
ethanolic extracts of Astraeus
hygrometricus and Tricholoma
giganteum, two wild edible
nutritious mushrooms, against carbon
tetrachloride (CCl4) induced hepatic
damage in mice.
Methods: Chronic
hepatotoxicity was induced by p.o.
introduction of CCl4 in paraffin
oil. Administration of ethanolic
extracts of A. hygrometricus and T.
giganteum and standard reference
drug silymarin for 7 consecutive
days simultaneously with toxicant
(CCl4) was done orally. Estimation
kits for glutamate pyruvate
transaminase (GPT), glutamate
oxaloacetate transaminase (GOT),
total and direct bilirubin, and
alkaline phosphatase (ALP) were
purchased and used following the
manufacturer’s protocol. Serum was
used for determination of GPT, GOT,
total and direct bilirubin, and ALP.
Liver was removed for the
determination of antioxidant enzymes
and histopathological observations.
Statistical analyses was performed
by student’s ‘t’ test and in all the
cases results are mean SD of at
least 3 individual experimental
data.
Results:
Administration of ethanolic extracts
of A. hygrometricus and T. giganteum
actively lowered the CCl4 mediated
elevation of serum transaminases
such as GPT and GOT, total and
direct bilirubin, and of serum ALP
which were comparable with silymarin.
The hepatic antioxidant enzyme
levels such as superoxide dismutase,
peroxidase were reduced in the CCl4
treated animals. Administration of
extracts to CCl4 challenge restored
the hepatic antioxidant status to
such a level that they were close to
normal and silymarin treated sets.
Furthermore, histopathological
analysis of mice liver injury such
as severe necrosis, fatty
infiltration, fibrosis and
lymphocyte infiltration induced by
CCl4 indicated that our extracts
could significantly suppress the
toxicity induced by CCl4 in mice
liver.
Conclusion: The
findings suggested that ethanolic
extracts of A. hygrometricus and T.
giganteum protected CCl4 induced
chronic hepatotoxicity in mice by
restoring the liver antioxidant
status and they might be
incorporated in regular human
dietary regimen in order to obtain
the benefits of nature.
Acknowledgement:
The authors are thankful to Council
of Scientific and Industrial
Research (CSIR), Govt. of India for
providing financial assistance.
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