15th World Congress Clinical Nutrition

19th – 22nd September 2010  El Sokhna Resort -  Egypt

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Effects of MELISSA OFFICINALIS on liver oxidant / antioxidant status and serum glucose levels in Streptozotocin – induced diabetic rats

H Ozturk1, B. Ozturk2, N. Sevinc1, I. Durak1

1Ankara University Faculty of Medicine, Department of Medical Biochemistry, Ankara, Turkey.
2Selcuk University, Selcuklu Medical Faculty, Department of Biochemistry, Konya, Turkey.

 Objective: Consumption of melissa officinalis (MO) as a herbal tea has been traditionally advised to diabetic patients in Turkey for its anti-diabetic effect. There are also some studies reporting on the antioxidant content and effects of this herb. In this study, our aim was to establish the effects of MO on serum glucose levels and oxidant/antioxidant status of liver tissue in streptozotocin (STZ)-induced diabetic rats.

Methods: Twenty-five rats were included in the study. In order to induce diabetes, 60mg/kg STZ were administered to 20 of the animals, intraperitoneally. Eleven of the STZ-treated rats were excluded from the study (8 were died and 3 were not diabetic). Groups were arranged as; I-Controls (n=5), II-Diabetes mellitus (n=4) and III-Diabetes mellitus+melissae officinalis (n=5). MO supplementation (200 mg/kg/day) to diets of 5 hyperglycemic rats were started 7 days after STZ treatment and continued for 4 weeks. Serum glucose and liver malondialdehyde (MDA) levels and activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and xanthine oxidase (XO) enzymes were measured.

Results: Serum glucose level (454.637.5 vs. 547.034.8; p=0.022) and liver tissue CAT (71.411.8 vs. 86.76.5) and XO (0.260.05 vs. 0.330.10) activities were found to be increased in Group III compared to Group II. There were no significant differences with respect to liver tissue MDA levels and GSH-Px activities between all three groups. Liver tissue SOD (142.5184 vs. 86.813.0 and 99.4±7.7) and XO (0.540.07 vs. 0.260.05 and 0.33±0.10) activities were found to be decreased in both diabetes groups (Groups II and III) compared to controls (Group I).

Conclusions: In this study, MO did not exert any antidiabetic effect. MDA levels pointed out that induction of diabetes via STZ treatment does not have any oxidative effect on liver tissue, also. On the other hand, STZ treatment lowered both SOD and XO enzyme levels.


 
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