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15th
World Congress Clinical Nutrition
19th
– 22nd September 2010 El Sokhna Resort - Egypt
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Copyright © 2010.
WCCN2010.COM
All rights reserved |
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The chemo-protective effect of
turmeric , clove, chili and cardamom
on correcting iron overload-inducing
liver injury and other biochemical
alterations in rat models.
Eman A.Sadeek and Fatma H.Abd El-Razek
Biochemistry & Nutrition Depart ,
Women's College –Ain –Shams Univ,
Egypt
dr_emansaddeek@yahoo.com
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Aim of the work
Assessing the chemoprotective effect
of turmeric, clove, chili and
cardamom on correcting iron overload
-inducing liver injury in rat
models.
Methods: Design of the study :
Sprague Dawley rats weighing 145.8 g
were divided into 6 groups. The
first group served as a control and
consumed a standard diet containing
1 % mineral mixture ( AIN – 93). The
other 5 groups were injected once
intraperitoneally with sterile
ferrous saccharate solution
providing 30 mg Fe / kg body
weight). One group was kept without
further treatment and served as a
positive control. Groups 3, 4, 5 and
6 consumed diets to which finely
ground turmeric, clove, chili and
cardamom flour were incorporated at
2 % , respectively. The feeding
trial continued for 28 days. At the
end of the experiments, blood
samples were collected , the animals
were sacrified and the liver was
separated and saved frozen for
subsequent biochemical analysis.
Laboratory
investigations consisted of twelve
biomarkers to test the magnitude of
liver toxicity and the antioxidant
potential of the supplements. The
serum enzymatic assays for liver
function test [ALT, AST, ALP]. The
complete lipid profile in the serum
was analyzed using appropriate
enzymatic assays . In addition, the
indices of lipid peroxidation; the
thiobarbituric acid and the
antioxidative catalase enzyme
activity were measured. The liver
iron content was determined after
wet ashing.
Results: The iron
overload was associated with
significant mean increases in the
activities of the liver enzymes AST,
ALT, ALP (P< 0.05) compared with the
respective mean control values. All
parameters of hepatic lipid profile
, i.e., serum total cholesterol ,
triacylglycerol, LDL-cholesterol,
phospholipids, total bilirubin, MDA
showed significant increase. On the
other hand, the mean HDL –
cholesterol and the activity of
serum catalase were lower than the
respective mean values of the
control. Liver iron deposition also
increased significantly after the
iron overload. The incorporation of
the turmeric , clove, chili, or
cardamom in the diet at 2 %
significantly restored the serum
enzyme activities of the liver
function ; AST, ALT, ALP to normal
level. The mean values of lipid
profile and the MDA were also
reduced. The liver iron deposition
was reduced with significant
increase in the activity of mean
serum catalase and HDL-cholesterol
compared with the respective mean
values obtained with the positive
control overloaded with iron.
Conclusion : The observed
improvement in the liver functions
suggests that the chemo-protective
effect of the flavonoids present in
turmeric , clove, chili and cardamom
. Flavonoids are known to chelate
iron and the flavonoid – iron
complex is excreted . This result
may find application among
populations at risk of iron overload
; either acquired or inherited .
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