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15th
World Congress Clinical Nutrition
19th
– 22nd September 2010 El Sokhna Resort - Egypt
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Copyright © 2010.
WCCN2010.COM
All rights reserved |
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Gastroprotective effect of compounds
from some medicinal plants against
indomethacin induced ulcer
Sahar Salah a, Abdel
Razik Hussien Farrag b
Departments of Chemistry of Natural
Compounds a & Pathology
b, National Research Centre
,Giza , Egypt
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Objectives : The
study aimed the investigation of the
anti-ulcerogenic activity of
Matricaria chamomile flower heads,
cassia fistula pod and
identification of the bioactive
compounds
Materials: Dried
powder of Cassia fistula pods and
Matricaria chamomile flower heads
i-Ethanolic extracts: 250 g of each
medicinal plant were separately
extracted with 70 % ethanol. The
crude extract was evaporated and the
residue was saved frozen for the
subsequent biological evaluation.
ii- Gastroprotective activity :
Design of the experiment : Male
albino rats (body weight 150 g )
were deprived of food for 24 hrs
prior to the experiment but allowed
free access of drinking water .
Induction of lesions: The crude
extracts of cassia and chamomile
were suspended in 0.1 % Tween 80
solution and administrated orally to
a group of 8 rats at a dosage of 500
mg / kg body wt. One hour later, the
rats received an oral dose of 25 mg
indomethacin dissolved in 0.1 %
Tween 80 solution / kg body weight.
The control group received 25mg
indomethacin /kg . Four hour after
the administration of indomethacin ,
the rats were sacrified and their
stomachs were removed. Formol saline
( 2 % v/v ) was then injected into
the totally ligated stomachs for
storage over night .The next morning
, the stomachs were opened along the
greater curvature , then washed with
fresh 2% formol saline for the
visible counting of the number of
ulcers. Inhibition of ulcer lesions
is expressed as percentage value.
For pathological investigation the
stomachs were preserved in 10 %
formalin solution. Results: The oral
treatment with indomethacin resulted
in the formation of stomach ulcer
averaging in the control group. Oral
administration of Cassia fistula
pods and chamomile flowers inhibited
the formation of ulcers by 82 and 62
% , respectively. Higher purified
extracts obtained by bioguided
fractionation of Cassia fistula with
chloroform and fractionation of
Matricaria chamomile with ethyl
acetate inhibited the ulcer
formation by 89 – 94 % respectively
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The bioactive fractions were
identified by column chromatography
and spectroscopy.The following
compounds were identified rhein from
chloroform fraction of Cassia
fistula. Major constituents of ethyl
acetate fraction of Matricaria
chamomile were apigenin 7-[O-
glucoside] , apigenin 7-[O- ( 6''-
acetyl ) galactoside], apigenin
-7-[O-(6''- acetyl ) glucoside and
7-methoxyapigenin-4'-O–β-L-arabinofuranoside.
Pathological examination supported
and confirmed the same observations.
Conclusion : Rhein from Cassia
fistula pods and apigenin 7-[O-
glucoside] from of Matricaria
chamomile flowers exhibit
significant gastroprotective effects
against indomethacin induced ulcers.
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